EMT of ARPE-19 cells induced by TGF-β1.

<p>ARPE-19 cells were grown on glass coverslips for 24 hr, starved for 24 hr, and then incubated for 24 hr, 48 hr, 72 hr with 10 ng/ml TGF-β1. Compared with control cells (A, E, I, M, Q), stimulated cells displayed an altered mesenchymal morphology by phase-contrast microscopy (B, C, D), with decreasing expression of E-cadherin (F, G, H) and ZO-1 (J, K, L) and an increasing expression of fibronectin (N, O, P) and α-SMA (R, S, T) by immunofluorescence microscopy. The green signal represents the staining of corresponding protein, and the red signal represents the nuclei staining by DAPI. Original magnifications 100× (A–D); 400× (E–T).</p

The properties of the three newly identified peptides.

<p>The properties of the three newly identified peptides.</p

Demographic and clinical characteristics of 109 patients with diabetic retinopathy.

<p>DR, diabetic retinopathy; SD, standard deviation; CI, confidence interval; BCVA, best-corrected visual acuity; DM, diabetes mellitus; NPDR, non-proliferative diabetic retinopathy; PDR, proliferative diabetic retinopathy; DME, diabetic macular edema.</p><p>Demographic and clinical characteristics of 109 patients with diabetic retinopathy.</p

Predictors of time trade-off and rating scale utility values from diabetic retinopathy patients and ophthalmologists, determined by bivariate analyses.

<p>DR, diabetic retinopathy; TTO, time trade-off; RS, rating scale; DME, diabetic macular edema.</p><p>*Pearson correlation coefficients and analysis of variance (ANOVA) were used.</p><p>Predictors of time trade-off and rating scale utility values from diabetic retinopathy patients and ophthalmologists, determined by bivariate analyses.</p

Comparison of the time trade-off and rating scale utility values from patients and ophthalmologists.

<p>TTO, time trade-off; RS, rating scale; SD, standard deviation; CI, confidence interval.</p><p>* <i>p</i> value comparing the TTO and SG methods within each visual group using the paired two-tailed Student’s t test.</p><p>Comparison of the time trade-off and rating scale utility values from patients and ophthalmologists.</p

Comparison of the patients’ and ophthalmologists’ utility values using time trade-off and rating scale methods.

<p>TTO, time trade-off; RS, rating scale.</p><p>*t and <i>p</i> value comparing patients’ and ophthalmologists’ utility values within each visual group using the paired two-tailed Student’s t test.</p><p>Comparison of the patients’ and ophthalmologists’ utility values using time trade-off and rating scale methods.</p

Deep multiple instance learning for automatic detection of diabetic retinopathy in retinal images

This code is about the deep multiple instance learning for automatic detection of diabetic retinopathy (DR) in retinal images. We hope more people can get involved in the research field of weakly supervised learning for DR detection

The correlation between endogenous expression of Snail and E-cadherin in ARPE-19 cells.

<p>(A) Compared to HGF cells, ARPE-19 cells showed the typical epithelial morphology. Original magnifications 100×. RT-PCR (B) and Western blot (C) analysis of Snail and E-cadherin in ARPE-19, MCF-7 and HGF cells at the mRNA and protein levels respectively. MCF-7 cells were used as a positive control for E-cadherin expression and HGF cells were used as a positive control for Snail expression.</p

Western blot analysis of protein levels of NF-κB p65.

<p>(A) The total and phosphorylation levels of NF-κB p65 was analyzed by Western blot in ICB and retina complex of EIU rats for indicated periods. Lane 1: control; lane 2: LPS and vehicle; lane 3: LPS and PAPS (10 µg/eye); lane 4: LPS and PAPep (10 µg/eye). (B) RAW264.7 cells were pretreated with PAPep (1, 10, 50 µM) or PAPS (50 µM) for 1 h, then stimulated with LPS (100 ng/ml) for 30 min. Total and phosphorylation levels of NF-κB p65 was analyzed by Western blot. Lane 1: control; lane 2: LPS; lane 3: LPS and 50 µM PAPS; lane 4: LPS and 1 µM PAPep; lane 5: LPS and 10 µM PAPep; lane 6: LPS and 50 µM PAPep. (C) HUVEC were incubated with PAPep (1, 10, 50 µM) or PAPS (50 µM) for 1 h, then stimulated with TNF-α (10 ng/ml) for 30 min. Total and phosphorylation levels of NF-κB p65 was analyzed by Western blot. Lane 1: control; lane 2: TNF-α; lane 3: TNF-α and 50 µM PAPS; lane 4: TNF-α and 1 µM PAPep; lane 5: TNF-α and 10 µM PAPep; lane 6: TNF-α and 50 µM PAPep. ##, P<0.01 compared with control group, **, P<0.01 compared with LPS or TNF-α group. Data are expressed as mean±SD of three independent experiments, each performed in duplicates.</p

Migration after Snail knockdown in ARPE19 cells with or without TGF-β1 treatment.

<p>(A) Cells transfected with control-shRNA or Snail-shRNA were allowed to migrate transwell chambers for 18 hr in the presence or absence of TGF-β1. After 18 hr, the migrated cells were fixed, stained, and photographed. (B) The number of migrated cells. Data shown represent the average of three independent experiments. *P<0.05, compared with control-shRNA, TGF-β1(−) samples and control-shRNA, TGF-β1(+) samples.</p